RESUMO
Background: in this study, we compared the effect of ibuprofen [IB] while incorporating by Poly Lactic-co-Glycolic Acid [PLGA] nanofiber on expression of adhesion molecules ICAM-1 and VCAM-1 in a mice adhesion model
Materials and Methods: using an adhesion model were induced in mice, PLGA-IB and PLGA membranes and IB were sutured between the abdominal wall and peritoneum after surgical operation to reveal the best membrane for prevention of postoperative adhesion bands by comparison of ICAM-1 and VCAM-1 expression
Results: compared with other groups, PLGA-IB showed a greater ability to reduce ICAM-1 and VCAM-1 expression
Conclusion: these results suggested that in considering the FDA approved polymers, PLGA-IB could be introduced as a potential candidate for prevention of abdominal post-surgery inflammation and adhesion band formation after surgeries
RESUMO
Assessments of cell reactions such as motility, orientation and activation to the topography of the substratum will assist with the fabrication of a proper implantable scaffold for future tissue engineering applications. The current challenge is to analyze the orientation effect of elecrospun nanofibers of poly [epsilon-caprolactone] [PCL] on viability and proliferation of mouse embryonic stem cells [mESCs]. In this experimental study, we used the electrospinning method to fabricate nanofibrous PCL scaffolds. Chemical and mechanical characterizations were specified by the contact angle and tensile test. O2 plasma treatment was used to improve surface hydrophilicity. We used the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide [MTT] assay to evaluate mESCs adhesion and proliferation before and after surface modification. The influence of the orientation of the nanofibers on mESCs growth was evaluated by scanning electron microscopy [SEM]. Statistical analysis was performed using one-way analysis of variance [ANOVA] With differences considered statistically significant at p